Introduction. The severity of the current epidemic situation is aggravated by the constant increase in the proportion of people with HIV infection in the population. The bacteriological characteristics of the pathogen isolated from patients with HIV‐associated tuberculosis confirm the high probability of an unfavorable prognosis: sensitivity to anti-tuberculosis drugs was detected only in 17.9% of patients, whereas in 82.1% of patients with drug-resistant tuberculosis, the most severe variants in the form of broad drug resistance (41%), multidrug resistance (47%), whereas mono‐ and polyresistance accounted for 3% and 9%, respectively. Aim. To compare the results of testing sensitivity/resistance to rifampicin and other anti-tuberculosis drugs using molecular genetic methods and phenotypic tests of MBT isolates isolated from clinical material of tuberculosis patients with HIV infection. Materials and methods. The article presents the results of the analysis of medical records with a diagnosis of pulmonary tuberculosis. A retrospective study was conducted of 176 histories of inpatient patients diagnosed with pulmonary tuberculosis with concomitant HIV infection - patients of the phthisiological department of the BUZ UR "RCTB MH UR" for the period January to December 2023, who underwent sputum testing for tuberculosis microbacteria (MBT) by isolating MBT cultures on dense (Levenstein-Jensen) and liquid nutrient media (VASTES MGIT 960). Additionally, sputum was tested by real-time polymerase chain reaction (PCR RV). Results. When comparing the results of testing sensitivity/resistance to rifampicin and other anti-tuberculosis drugs by molecular genetic methods and phenotypic tests of MBT isolates isolated from clinical material of tuberculosis patients with HIV infection in sample populations, it was revealed that all three methods coincided only in 20 people out of 176 studied - 11.36±2.4%, and in tuberculosis patients with HIV infection, including patients with XDR who secrete resistant MBT to rifampicin and isoniazid, to fluoroquinolones, as well as bedaquiline or linezolid according to PCR results, there are no PPP for registered cases, according to Bactec results - 97 cases (55.11±7.40%), which, in turn, confirms the high the sensitivity of the Bactec MGIT 960 stability assessment method. Conclusion. Based on the sample and its study, it was revealed: the coincidence of the analysis results between BACTEC, the classical method, and the PCR method was 11.36±2.4%. In turn, this proves that none of the methods of modern diagnostics for determining LF MBT is the "gold standard". According to the results of a retrospective analysis, the most sensitive method for assessing the stability of MBT was the Bactec MGIT 960 method.