Introduction (relevance). The health and lifestyle of adolescents are formed under the influence of many interrelated factors, and the social environment plays a decisive role in this process. Аim: study of socio-cultural factors affecting the formation of adolescents' behavior in relation to their health Materials and Methods. Results.Discussions. In 2023, a one-stage survey descriptive study was conducted using the questionnaire method of 930 adolescents studying in medical pre-professional educational programs. The median age was 16.29±0.88 years. The questionnaire included questions assessing the terminal values of adolescents, awareness of key aspects of health and risks, questions about family traditions related to health care. Results.The most significant values for adolescents were: love (41.4%), health (23.1%), financially secure life (16.8%). Slightly more than 37% of the surveyed adolescents noted the presence of well-established health-saving traditions in the family, on average, 31% noted that they were not familiar with the possible health risks associated with the use of alcohol, drugs and low physical activity. Discussions. The study confirms the theoretical conclusions about the dominant values and behavioral orientations of adolescents of this age period and is due to psychophysiological characteristics and the influence of society. A significant number of adolescents do not show initiative in health-saving behavior, which indicates the need to develop additional programs and activities aimed at encouraging positive behavior in relation to their health in schoolchildren. Conclusions (conclusion). Despite the fact that the socio-cultural aspects affecting adolescents are actively transforming in modern conditions, the factors influencing the formation of adolescents' behavior in relation to their health still retain the traditional social basis, which includes the family, the society of peers, as well as educational institutions.