Introduction. Type 2 diabetes mellitus in many countries is simultaneously accompanied by an increase in the frequency of diabetic retinopathy, which is the most dangerous complication of type 2 diabetes mellitus, leading in some cases to complete loss of vision. Biological age may be one of the contributing factors of diabetic retinopathy in type 2 diabetes mellitus. The aim of the study to study the correlation of biological age with biomarkers of anabolic and catabolic hormones in lacrimal fluid and to create mathematical models to support decision-making on screening for diabetic retinopathy in type 2 diabetes mellitus. Material and methods. The chronological age was recorded in 435 patients included in the study, and then the biological age was calculated according to the Voitenko V.P. method, taking into account gender. The concentration of the studied hormones in the lacrimal fluid was determined by high-performance liquid chromatography in compliance with all necessary methodological techniques. The statistical processing used the program «Statistica 10.0», nonparametric criterion X2 and discriminant analysis. Results. The biological age of patients 45-59 years old with DR in type 2 diabetes in the group with an excess of the biological age of the chronological value, according to the results of correlation analysis, is associated with the content of cortisol, dehydroepiandrosterone, adrenaline and dopamine in the lacrimal fluid in the morning with a direct reliable average correlation. In addition, the calculated parameters of the coefficients of multidimensional regression analysis revealed the heterogeneity of groups of patients with the presence of dissociation between the parameters of calendar and biological age with advanced DR in type 2 diabetes, which indicates the influence of biological age on differences in the content of catabolic and anabolic hormones in the salivary fluid in the morning of patients 45-59 years old with advanced DR due to diabetes type 2, and the acceleration of biological age due to these changes contributes to the development of DR due to type 2 diabetes. An analysis of the effectiveness of the created discriminant models in terms of the content of cortisol, adrenaline, dopamine in the lacrimal fluid in the morning and the value of biological age in accordance with the Mahalanobis distance revealed the erroneous inclusion of patients 45-59 years old with the presence of dissociation between the parameters of calendar and biological age with developed DR due to type 2 diabetes in the cohort of mature age with the absence of dissociation between the parameters calendar and biological age at 13,89%. Conclusion. The created mathematical models can be used in clinical activities when performing screening measures taken taking into account the results of the created models, with sufficient efficiency. The determination of the diagnostic sensitivity of the models discussed above showed that it is equal to 0,87, and the diagnostic specificity is slightly lower – 0,85.