Introduction. Diabetic retinopathy (DR) is a common microvascular complication of diabetes mellitus (DM) and remains the leading cause of preventable blindness among working-age individuals. The underappreciated contribution of aging to the development of DR requires further study. The aim of the study to determine markers of acceleration of biological age in patients aged 45-59 years with diabetic retinopathy due to type 2 diabetes mellitus and to construct discriminant models based on the data obtained. Material and methods. Biological age was calculated using the method of Voitenko V.P. When diabetic retinopathy was detected, key points in the medical history were recorded, including an ophthalmological history. In the morning (8.00-9.00 a.m.), content of catabolic and anabolic hormones in the urine were determined. Objective assessment was carried out using various mathematical methods - correlation, multivariate regression, discriminant analysis, indicators of diagnostic sensitivity and specificity. The results of the study. The presence of pronounced correlations between catabolic and anabolic hormones in a group of patients with an ophthalmological complication induced by type 2 DM, aged 45-59 years, indicates the influence of the dissociation of passport and biological age on the development of DR. Determining the coefficients of multivariate regression analysis revealed that among the studied hormones of premature aging and body temperature in the morning, cortisol, melatonin, adrenaline and dopamine have statistical significance. In this regard, data processing using the discriminant method included only hormones that retained statistical significance - melatonin, adrenaline and dopamine - and discriminant models were built. Determining the quality of differentiation of the two compared groups based on the Mahalanobis distance showed that discriminant models created taking into account the relationships between melatonin, adrenaline and dopamine content and biological age can be used to screen for accelerated biological age in patients with DR due to type 2 DM. Conclusion. Melatonin, adrenaline and dopamine are markers of accelerated biological age in patients aged 45-59 years with DR due to type 2 DM.