Significance. Spread of HIV-infection and blood-borne viral hepatitis is a serious issue of modern global healthcare. Priority should be given to preventive measures aimed at reducing the risk of infection and promoting a tolerant attitude towards patients living with these infections as well as to treatment and diagnostics. Preventive measures can be carried out deliberately if the awareness levels on the issue are known. Purpose. To evaluate the level of awareness on HIV-infection and blood-borne viral hepatitis among the population of the Magadan oblast, to detect priority area of preventive work aimed at reducing the spread of infections. Materials and methods. An anonymous survey on a voluntary bias was carried out according to the questionnaire developed by the authors that includes 16 questions, number of answers range from 3 to 8. The surveillance was performed utilizing Google-form platform in the telecommunication network Internet among the population of the Magadan oblast (n=769). Statistical evaluation of obtained results was performed with Microsoft Excel 2013 software. Results. False statements and existing myths about the spread of HIV and blood-borne viral hepatitis were revealed (blood-sucking insects bite – 19.9%, kiss – 11.7%, food sharing – 7.8%) despite overall satisfactory awareness of the population on the issue of HIV and blood-borne viral hepatitis spread. Stigmatizing behavior towards people living with HIV and blood-borne viral hepatitis was revealed: 18.5% experience fear and tension, 24.4% doubt they would shake hands with an infected person. The survey showed unsatisfactory knowledge concerning prevention measures on using condoms and undergoing regular medical checkups. Risk of sexual transmission remains high due to neglecting use of condoms during sexual intercourse (51.5% use condom). Internet and medical staff (78.9% and 64.2% respectively) are reputable sources of information about the topics among the surveyed population. Obtained results should be considered as an original indicator for defining the area of preventive work. Given the received data, focus on legal aspects of HIV-infection and blood-borne viral hepatitis, preventive measures against HIV-infection and blood-borne viral hepatitis, regular checkups of the population should be taken into account when developing preventive strategies. Conclusion. The aim of the research was reached: awareness levels on HIV-infection and blood-borne viral hepatitis among the population was evaluated, priority area of awareness-raising and preventive work was defined, practical recommendations were specified. Scope of Application. Up to date regional preventive measures program aimed at reducing the risk of acquiring HIV and blood-borne viral hepatitis among the population of the Magadan oblast is to be developed/corrected according with the obtained results.