The aim of this study was to research component composition and antimicrobial activity of Cichorium intybus herb infusion. The study object was Cichorium intybus herb. It was harvested in plant natural habitat in mass flowering phase. The component composition of Cichorium intybus herb infusion was studied by liquid chromatography method. 8 phenolic compounds were found, chlorogenic acid and luteolin-7-O- glucoside were identified. Ratio hydroxycinnamic acids and flavonoids in the infusion was 1: 1. Antimicrobial activity of Cichorium intybus herb infusion was studied by serial dilution method. Inhibitory effect of the studied dosage form was noted towards Candida albicans (ATCC 10231) at the dilution of 1: 10. Infusion concentration decrease reduced the activity of the dosage form in regard to this strain.